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SALT LAKE CITY, Utah – A unique collection of tissue samples donated by people with chronic myeloid leukemia was the driving force behind the discovery of a potentially major new treatment approach for drug-resistant leukemia. this week in the Proceedings of the National Academy of Sciences (PNAS) of the United States of America, researchers report how a laboratory study of a specially engineered formulation of gas, composed of charged and highly reactive molecules, called "cold atmospheric plasma," or CAP, was highly effective in targeting drug-resistant leukemia cells while protecting nearby healthy cells.
There are many types of leukemias that arise in bone marrow cells. While research has led to major improvements in the survival of adult and child leukemia patients, some leukemias that were at one time responsive to treatment become resistant to the drugs used to target them. Therefore, scientists are working to improve treatment options for leukemia patients by understanding the characteristics of aggressive leukemias that are more likely to develop drug resistance.
The study was powered by a unique resource at Huntsman Cancer Institute (HCI) at the (U of U) called the Division of Hematology Biorepository. This resource provided patient-donated leukemia samples with the precise characteristics needed to conduct the study to determine how CAP blocks strategies leukemia cells use to survive.
Study co-first author Tony Pomicter, MS, leads the team that runs the biorepository. "Our entire team of study coordinators, specimen processors, database specialists, and healthcare providers spent many years collecting thousands of samples donated by thousands of patients at Huntsman Cancer Institute so we could have just the right diagnosis, cell type, cell count, treatment histories, and mutations for this study," he said.
Pomicter credits the critical partnership between patients, doctors, and laboratory scientists in driving cancer research discoveries forward. In 2019 alone, more than 450 patients donated samples to the biobank. Collected samples are tied to the patient’s medical record, providing a high degree of precision on the unique characteristics of the cells at the time of collection; for example, whether the sample is prior to treatment or after a relapse.
The HCI team was approached for samples from the biorepository by and Hai-Lan Chen, PhD, the corresponding authors of the study and professors at Old Dominion Ï㽶ÊÓƵ, Norfolk, Virginia. Kong, a biomedical engineer, and Chen, a molecular biologist, were working on understanding how CAP could be tailored to specifically target drug-resistant leukemia cells. Previous work demonstrated anticancer activity of CAP by containing or generating chemical and physical effectors. Kong and Chen noted that the biological effectors in certain CAP preparations can activate cancer cell survival and actually make the cells drug-resistant, so they knew that studies on cancer cells taken directly from patients would be key to understanding how to formulate CAP to instead prevent cancer cell survival and drug resistance.
Kong, Batten Endowed Chair in Bioelectrics at Old Dominion Ï㽶ÊÓƵ, reflects how the journey to the discovery started in a different field. "In our work in developing CAP to overcoming drug-resistant
microbial pathogens, we noted that an excellent antimicrobial activity of CAP is centered on the cooperation of its many components," he said. "We reasoned that potent anticancer activity may be achieved by engineering such cooperation of CAP’s many components at very low doses to kill cancer cells. To our surprise, the strategy of leveraging the cooperation of diverse, low-dose reactive oxygen species, ions and other effectors actually enables simultaneous blockade of three fundamental cancer cell resistance pathways, leading to a very high-rate of death in drug-resistant leukemia cells."
Kong cites the central importance of the interdisciplinary cross-fertilization between gas plasma physicists, biomedical engineers, and molecular biologists as well as the catalyst of working with Huntsman Cancer Institute.
Chen, Research Associate Professor in Molecular Biology, agrees. "Chronic myeloid leukemia is an exceptional model to test CAP since it is caused by a single molecular event and the mutations are well understood," Chen said. "Efficacy data with chronic myeloid leukemia cells allowed us to establish a clear picture of the underlying mechanisms with little risk of ambiguity. We look forward to future collaborations with Huntsman Cancer Institute to take this work forward on leukemia and other cancers."
Kong and Chen worked with the HCI team to identify the specific samples from the biorepository for study. Using the detailed records the team has developed for its biorepository, they identified blood samples with the most difficult-to-treat mutation, as well as samples from healthy donors. These leukemia and healthy cells were cultured in the lab so the team could conduct experiments using different formulations of CAP. "It was critical to evaluate healthy cells alongside leukemia samples," says Pomicter. "We always need to understand whether a novel agent is killing all cells, which means it is a toxin, or selectively killing cancer cells, which means it is potentially a drug."
The team found that the cold atmospheric plasma appears to be effective at killing the leukemia cells, but not the healthy cells, making it a promising approach for possible future use in the clinic on other difficult-to-treat hematologic malignancies like acute myeloid leukemia and acute lymphoblastic leukemia.
The researchers acknowledge the critical role of all study authors, including co-first author Bo Guo, PhD, who helped to formulate Trident CAP and performed experiments. Michael Deininger, MD, PhD, at HCI at the time of the study and now executive vice president and chief scientific officer at Versiti Blood Research Institute, provided critical leadership in the expansion of the Division of Hematology Biorepository as well as other research direction in the study. Other study authors include Francis Li, Sudhir Bhatt, Chen Chen of Old Dominion Ï㽶ÊÓƵ; and Wen Li, Miao Qi, Dei-Hui Xu, and Chen Huang of Xi’an Jiotong Ï㽶ÊÓƵ in China. HCI’s Research Informatics and Biorepository and Molecular Pathology Shared Resources were instrumental in the work. The study was funded by the National Institutes of Health/National Cancer Institute including R01 CA178397, P30 CA042014, Huntsman Cancer Foundation, and Batten Endowed Chair Fund at Old Dominion Ï㽶ÊÓƵ.
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About Old Dominion Ï㽶ÊÓƵ:
Old Dominion Ï㽶ÊÓƵ, located in Norfolk, is Virginia’s forward-focused public doctoral research university with approximately 23,500 students, rigorous academics, an energetic residential community and initiatives that contribute $2.6 billion annually to Virginia’s economy.